仙客来播种育苗试验

将仙客来种子用高锰酸钾质量分数1000×10 6液浸泡26h左右,进行种皮软化和消毒处理,然后播种在泥炭基质内,深度为(0 6±0 1)cm。在无光条件下保持温度(20±5)℃,湿度90%左右,在50～60d后即可发芽。发芽后应注意增加光照和施肥,也可以用稀释的肥料代替喷水,成苗率达89 9%。     

不同光环境下水曲柳幼树光合指标的生理反应

应用Li-640002B型光合仪对生长在不同光环境下的水曲柳幼树的光合特性进行了测定。统计分析结果表明,幼树对低光环境具有较强的适应能力,能够在0.512μmol·m-2·s-1的条件下正常生活。幼树光合速率最高可达13.0μmol·m-2·s-1,光饱和点(LSP)1000μmol·m-2·s-1左右,适生光环境为800～1000μmol·m-2·s-1。     

Effects of light and microsite conditions on tree size of 6-year-old Cryptomeria japonica planted in a group selection opening

We examined the extent to which direct and indirect measures of light and microsite conditions could explain variation in tree height and diameter at the base of 6-year-old Cryptomeria japonica trees planted in a group selection opening of about 0.32 ha on a steep slope at Shiiba, Miyazaki Prefecture, southern Japan. We first used the gap light index (GLI) and soil thickness (ST) as directly measured indices. For an indirect measure of light, we used a between-cohort competition index (BCI) estimated from the position and total height of residual trees. For indirect measures of microsite, we examined topographic indices (slope, plan and profile curvature, average slope gradient, and relative elevation) derived from digital elevation models (DEMs) with different resolutions ranging from 2 to 10 m. The multiple linear regression using GLI and ST explained about 45% of variation in tree size, while simple regression using only GLI explained about 35%. The contribution of ST was about half of GLI. The multiple regressions using BCI and the topographic indices did not explain any more variation than using BCI alone (R ² of about 0.26). We conclude that microsite conditions with shallower soil and steeper slope have negative effects on tree growth in group selection openings, although the relative importance is smaller than light conditions. More comprehensive studies considering several openings with more heterogeneous topography including different species are needed to generalize our growth prediction using the indirect measures, which are useful for practical forest management.     

光照条件对小报春生长及光合特性的影响

小报春Primulaforbesii是一种观赏价值高,生态适应性强,具有园林应用潜力的野生花卉.在将其引种后应用于园林的过程中,特别对其引种后在不同光照条件下的生长情况及光合特性进行了研究分析.结果表明:1)在大气CO2浓度下,光强是影响小报春光合作用的关键因子;2)小报春的光补偿点在26.18～43.10μmol·m-2s-1之间,饱和点低于1400μmol·m-2s-1;3)小报春植株有一定的耐荫性,不适合在强阳光直射的地方生长,种植应用时应给予一定的侧方遮荫.     

不同覆盖处理对元宝枫叶生物活性物质含量的影响

以2年生元宝枫苗为试材,对其进行不同覆盖处理,测定叶内黄酮、绿原酸、叶绿素、SOD活性酶和丙二醛的含量,并进行方差分析和多重比较.结果表明:不同覆盖处理对元宝枫叶中生物活性物质含量的影响十分明显,黄酮含量在白膜覆盖下最高,绿原酸和叶绿素含量在绿膜覆盖下最高,SOD酶活性和丙二醛含量在红膜覆盖下最高,黄酮、叶绿素、丙二醛含量在不同覆盖处理下均以α=0.01水平上差异极显著,SOD酶活性和绿原酸含量以α=0.05水平上差异显著.     

CaCl_2处理对奈李低温贮藏期生理生化的影响

为了探明 Ca2 对奈李低温贮藏的保鲜效应 ,对奈李采用不同浓度的 Ca Cl2 溶液处理、聚乙烯薄膜袋包装、在低温 ( 5℃ )下贮藏 ,研究其生理生化变化情况 .结果表明 :采用质量分数为 0 .5 %～ 1.0 %的 Ca Cl2 溶液处理 ,对奈李的呼吸作用、Vc含量的降低、可溶性固性物的消耗、电导率和 POD(过氧化物酶 )活性的升高以及贮藏期的失水等均具有抑制作用 ,而用较高浓度的 Ca Cl2 ( 2 .0 %～ 6.0 % )溶液处理 ,则具有相反的作用 .     

Cultivation of shiitake in sugi wood meal II: effects of seasoning treatment for wood meal on mycelial growth

The effects of seasoning treatment for fresh sugi wood meal on the mycelial growth of shiitake was investi-gated. The mycelial growth of shiitake in sugi wood meal increased to the same level as that in extract-free sugi wood meal on the 12th day and to approximately 70% of that in konara wood meal on the 28th day of the seasoning treatment. A drastic decrease in extracts of fresh sugi wood meal occurred during the early stage of treatment, and the neutral fraction of methanol extracts decreased to less than 2%. The drainage of sugi wood meal media significantly increased during the later stage of treatment. No changes in the chemical components of sugi wood meal were detected during the seasoning treatment. The mycelial growth of shiitake in the fully seasoned sugi wood meal, which was prepared for commercial mushroom cultivation, was at almost the same level as that in the extract-free sugi wood meal. All of the seasoned sugi wood meals contained a neutral fraction of less than 1%. These results suggested that seasoning treatment can promote mycelial growth of shiitake in sugi wood meal by eliminating inhibitors and improving the physical properties of sugi wood.Part of this paper was presented at the 49th Annual Meeting of the Japan Wood Research Society, Tokyo, April 1999     

Infrared spectroscopic study of alkaline oxygen treatment of lignin with ATR technique in aqueous state 1: method for determining quantitative spectra of oxygen-degraded lignin

For the fundamental study of oxygen delignification of kraft pulp, structural changes of kraft lignin during alkaline oxygen treatment were investigated with the use of infrared measurement with attenuated total reflectance (ATR) technique. In the neutralized reaction mixture of alkaline oxygen-treated kraft lignin, there is a significant amount of NaCl, so that the spectral changes of water due to the coexistence of NaCl was investigated, and how to remove the huge absorption of NaCl solution is discussed. Sodium vanillate–NaCl solutions were employed as model solutions for the reaction mixture. Partial least square (PLS) regression was applied for the prediction of NaCl concentration, and the spectrum of NaCl solution was subtracted from the spectrum of sodium vanillate–NaCl solution as background measurement. This allowed us to obtain the vanillate spectra free from the absorption of NaCl solution. In addition, the mathematical method for reconstructing the spectrum of NaCl solution is discussed. The spectrum of NaCl solution is reconstructed as the linear combination of basic spectra calculated by singular value decomposition (SVD), and it was subtracted from that of the sodium vanillate–NaCl solution. By this procedure, the vanillate spectra were also obtained quantitatively, as has been demonstrated in PLS regression study. It was also confirmed that the quantitative spectra of high molecular weight fraction of alkaline oxygen-treated kraft lignin were obtained by the use of this reconstruction technique.Parts of this report were presented at the 52nd Annual Meeting of the Japan Wood Research Society, Gifu, Japan, April 2002 and the 12th International Symposium on Wood and Pulping Chemistry, Madison, USA, June 2003     

Effects of myosin light chain kinase on regulation of endothelial barrier function

Myosin light chain kinase (MLCK) activates the regulatory light chain of myosin II, and the phosphorylated myosin light chain leads to actomyosin contractile activity, as well as the cell contraction and increasing intercellular gap, which finally results in endothelial barrier dysfunction. MLCK-dependent hyperpermeability occurs in response to multiple cell signaling molecules and signaling pathways, including Ca²⁺, Src, PKC, NO, cGMP and mitogen activated protein kinases (MAPK). In this review, different mechanisms of endothelial hyperpermeability mediated by MLCK are discussed.     

Effects of inhibiting myosin light chain kinase on endothelin-1 induced proliferation and apoptosis of pulmonary arterial smooth muscle cells in rats

AIM: To investigate the effect of inhibiting myosin light chain kinase(MLCK) on endothelin-1(ET-1) induced proliferation and apoptosis of rat pulmonary artery smooth muscle cells(PASMCs). METHODS: Rat PASMCs were cultured and stimulated with ET-1. The cells were randomly divided into control group, ET-1 group and ET-1+MLCK inhibitor group(ET-1+M). Western blotting, MTT assay, [³H]-TdR incorporation and flow cytometry were employed to test the expression of myosin light chain(MLC) and MLCK, cell proliferation, cell cycle and apoptotic rate of PASMCs,respectively. The phosphorylation of MLC was determined by glycerol-PAGE coupled with Western blotting. RESULTS: Compared with control group, the protein expression of MLCK and MLC phosphorylation significantly enhanced after ET-1 stimulation. ET-1 markedly induced the proliferation and decreased the percentage of apoptotic rate in the PASMCs. However, pretreatment with ML-7, a MLCK inhibitor, significantly reversed the above effects induced by ET-1. CONCLUSION: MLCK inhibitor effectively inhibits the ET-1-induced proliferation and the cell cycle progression.